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KMID : 0356219930240010068
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Journal of the Korean Military Medical Association
1993 Volume.24 No. 1 p.68 ~ p.75
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The Effect of Active Immunization with N. fowleri in Mice Born to Immune Mothers
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Abstract
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N. fowleri is a pathogenic free-living amoeba causing primary amoebic meningoencephalitis (PAME) in human and mouse. Several reprots on the immune responses in mice with this infection have published, but the effects
of transferred passive immunity
on
the protective effect ofactive immunization in offspring mice have demonstrated.
This study was done to observe the effect of active immunization with N. Fowleri in mice born to immune mothers. N fowleri was cultured in the GVS medium axenically. Female Balb/c mice weighing about 20g were immunized through the intraperitoneal
injection of N. fowleri 1×10E6 trophozoites each three times at the interval of one week. Offspring mice were immunized times with same methods.
The mice were inoculated intran??ally with 1×10E4 trophozoites under secobarbital anesthesia.
There was statistical differences in mortality between transferred immunity group and active immunization only group. We could not find statistical differences in antibody titer between both groups. But Ly2+T cell ratio increased in transferred
immunity
group more than active immunization only group. Active immunization was done to the offspring mice when it was 5 weeks old. There was no differences in nortality between both groups. It was recognized that active immunization procedures in
offspring
mice born to immune mother could alter the immune status according to its time of immunization.
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KEYWORD
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